Transwell Biotech Co., Ltd.

This is an animal-free epithelial cell culture medium – EpiV Medium

Transwell Biotech Co., Ltd.
Transwell Biotech Co., Ltd.

Product Description

EpiV Medium is a serum-free, Bovine Pituitary Extract (BPE)-free, Human Platelet Lysate (HPL)-free, cholera toxin-free, chemically-defined culture medium for the growth of epithelial cells and keratinocytes without the use of feeder layers, but not for the growth of fibroblasts. EpiV Medium does not contain any material sourced from animals or humans. EpiV Medium supports the primary culture of keratinocytes freshly isolated from tissues and the subsequent long-term culture.
Keratinocytes play a very important role in the process of wound healing. However, the cultivation of keratinocytes is quite difficult. Keratinocytes have a slow growth rate, very limited passage number, and are easy to differentiate. At present, the commonly used keratinocyte culture media on the market mostly contain BPE, which contains unknown ingredients and carries infection risk of Bovine Spongiform Encephalopathy (BSE). On the other hand, the cell culture performance of BPE- and serum-free keratinocyte culture media is usually too poor to quickly produce a large number of keratinocytes for clinical applications. Our company has obtained the exclusive license of EpiV Medium from the Medical and Pharmaceutical Industry Technology and Development Center (PDC), and then improved and optimized it by our R&D team. The cell attachment ability, growth rate, and passage number of keratinocytes in EpiV Medium are significantly better than other competitor media.
Transwell Biotech Co., Ltd.

Product Specification

Component
Catalog no.
Volume
Storage
Shelf life
EpiV Basal Medium
M001
500 mL
4°C ; Protect from light
12 months
EpiV Growth Supplement
S001
0.5 mL
–20°C ; Protect from light
12 months

EpiV requires addition of the EpiV Growth Supplement to the EpiV Basal Medium before use. The complete medium (i.e. supplemented medium) of EpiV should also be stored at 4°C in the dark without being frozen.

Transwell Biotech Co., Ltd.

Product Advantage

01 Primary human keratinocytes growth better in EpiV than other media on market.
Initial culture of isolated keratinocytes from tissue.
EpiV Medium
EpiV Medium
X-SFM
X-SFM
X-Life
X-Life
  • Cell:Human Foreskin Keratinocytes (P0)
  • Seeding density:4.0x 104 cells/cm2
  • Culture period:5 days
Keratinocytes in EpiV continuously grow well into high passages.
Cell Number Cell Number
  • Cell:Human Foreskin Keratinocytes (P9)
  • Seeding density:4x 103 cells/cm2
  • Culture period:5 days
  • CK14:Cytokeratin 14
02 Collagen coating is optional. Coating increases the attachment and proliferation of keratinocytes in EpiV medium. (Poly-L-Lysine and iMatrix-511 can be used instead of collagen)
Non-coated
Day3
Non-coated Day3
Day5
Non-coated Day5
Collagen-coated
Day3
Collagen-coated Day3
Day5
Collagen-coated Day5
Cell Number
  • Cell:Human Foreskin Keratinocytes
  • Seeding density:4x 103 cells/cm2
  • Culture period:5 days
03 Fibroblasts cannot proliferate in EpiV medium, avoiding fibroblast contamination during keratinocyte isolation from tissues.
Fold
  • Cell:Human Dermal Fibroblasts
  • Seeding density:1~4x 103 cells/cm2
  • Culture period:5 days
  • Fold:Day5 cell number/seeding cell number