EpiV Medium is a serum-free, Bovine Pituitary Extract (BPE)-free, Human Platelet Lysate (HPL)-free, cholera toxin-free, chemically-defined culture medium for the growth of epithelial cells and keratinocytes without the use of feeder layers, but not for the growth of fibroblasts. EpiV Medium does not contain any material sourced from animals or humans. EpiV Medium supports the primary culture of keratinocytes freshly isolated from tissues and the subsequent long-term culture.
Keratinocytes play a very important role in the process of wound healing. However, the cultivation of keratinocytes is quite difficult. Keratinocytes have a slow growth rate, very limited passage number, and are easy to differentiate. At present, the commonly used keratinocyte culture media on the market mostly contain BPE, which contains unknown ingredients and carries infection risk of Bovine Spongiform Encephalopathy (BSE). On the other hand, the cell culture performance of BPE- and serum-free keratinocyte culture media is usually too poor to quickly produce a large number of keratinocytes for clinical applications. Our company has obtained the exclusive license of EpiV Medium from the Medical and Pharmaceutical Industry Technology and Development Center (PDC), and then improved and optimized it by our R&D team. The cell attachment ability, growth rate, and passage number of keratinocytes in EpiV Medium are significantly better than other competitor media.
–20°C ; Protect from light
EpiV requires addition of the EpiV Growth Supplement to the EpiV Basal Medium before use. The complete medium (i.e. supplemented medium) of EpiV should also be stored at 4°C in the dark without being frozen.
Primary human keratinocytes growth better in EpiV than other media on market.
Initial culture of isolated keratinocytes from tissue.
Keratinocytes in EpiV continuously grow well into high passages.
Collagen coating is optional. Coating increases the attachment and proliferation of keratinocytes in EpiV medium. (Poly-L-Lysine and iMatrix-511 can be used instead of collagen)
Fibroblasts cannot proliferate in EpiV medium, avoiding fibroblast contamination during keratinocyte isolation from tissues.